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TDG Antibody
Polyclonal antibody to detect TDG in human, mouse and rat samples.
Product Overview
Product Name: TDG Antibody
Antibody Target: TDG
Alternative Name: TDG.
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Fusion protein of human TDG
Accession#: Q13569
Gene ID: 6996
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Species Reactivity: Human, mouse, rat
Application: Western blot, Immunohistochemistry
Positive Control: Western Blot: Brain cell lysates
Application & Usage: Western blot: 1:500 – 1:1000.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: In the DNA of higher eukaryotes, hydrolytic deamination of 5methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon nitrogen bond between the sugar phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitinlike proteins SUMO1 and SUMO2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |