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SMARCA4 Antibody
Polyclonal antibody to detect SMARCA4 in human, mouse and rat samples.
Product Overview
Product Name: SMARCA4 Antibody
Antibody Target: SMARCA4
Alternative Name: BAF190, BRG1, FLJ39786, SNF2, SNF2BETA, SNF2L4, SNF2LB, SWI2, hSNF2b.
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: A synthetic peptide of human SMARCA4
Accession#: P51532
Gene ID: 6597
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification: Affinity purified
Species Reactivity: Human, mouse, rat
Application: Western blot, Immunohistochemistry
Positive Control: Western Blot: Various cell lysates
Application & Usage: Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histoneDNA contacts by ATPdependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATPdependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM. This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant. SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle and differentiation. Brg1/hBRM has been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors. Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression including with HDACs, Rb and Tif1β. Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressors and Brg1 is mutated in several tumor cell lines.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |