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Peroxiredoxin 1 Antibody (9D2)
Monoclonal Antibody to detect peroxiredoxin I in human samples.
Product Overview
Product Name: Peroxiredoxin 1 Antibody (9D2)
Antibody Target: Peroxiredoxin 1
Alternative Name: Peroxiredoxin 1, MSP23, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2.
Host: Mouse
Antibody Type: Monoclonal
Isotype: Mouse IgG 1
Immunogen: Recombinant human protein purified from E.coli
Accession#: Q06830
Gene ID: 5052
Appearance: Colorless liquid
Concentration: 1 mg/ml
Formulation: 100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Purification: Protein G purified Antibody
Species Reactivity: Human
Application: Western blot,IP,ICC
Positive Control: IP analysis : HeLa cell lysate. IHC staining : HeLa cells
Application & Usage: Western blot: 2-4 µl/ml, IP: 1-2 µl, ICC: 20 µl/ml.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx I is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor alpha by regulating the intracellular concentrations of H2O2. Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive post mitotic motor neuron differentiation.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |