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sRANK Ligand Antibody
Polyclonal antibody to detect sRANKL in mouse samples
Product Overview
Product Name: sRANK Ligand Antibody
Antibody Target: sRANKL
Alternative Name: soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor)
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: E.coli derived Recombinant Murine sRANKL
Accession#: O35235
Gene ID: 21943
Appearance: Lyophilized powder
Concentration: N/A
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Purification: Affinity chromatography
Species Reactivity: Mouse
Application: Western blot, ELISA, Neutralization
Positive Control: ELISA: Recombinant msRANKL
Application & Usage: 1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant murine sRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of msRANKL (50.0 ng/ml), a concentration of 0.05 μg/ml of this antibody is required.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |