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IL-33 Antibody
Polyclonal antibody to detect IL-33 in human samples
Product Overview
Product Name: IL-33 Antibody
Antibody Target: IL-33
Alternative Name: NF-HEV
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: E.coli derived Recombinant Human IL-33
Accession#: O95760
Gene ID: 90865
Appearance: Lyophilized powder
Concentration: N/A
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Purification: Affinity chromatography
Species Reactivity: Human
Application: Western blot, ELISA, Neutralization, Immunohistochemistry
Positive Control: ELISA: Recombinant hIL-33 (Cat # 4193). IHC: Human breast malignant ductal adenocarcinoma
Application & Usage: 1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/ml), a concentration of 0.2-0.4 μg/ml of this antibody is required.
4) Immunohistochemistry: 0.125 μg/ml-0.25 μg/ml with an overnight incubation at 4°C
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Human IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans. |