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Anti-Dynamin related protein 1 (Drp1)
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Ox, Rhesus Macaque WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Dynamin related protein 1 (Drp1) Antibody
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Specificity:
This antibody recognizes Dynamin related protein 1 (Drp1) at the GSK3B interaction domain.
Molecular Weight:
~80kDa (isoform 2) & ~82 kDa (isoform 1) observed
5 isoforms are produced by alternative splicing: Isoform 1: 80 kDa, Isoform 2: 82 kDa, Isoform 3: 78 kDa, Isoform 4: 69 kDa, and Isoform 5: 24 kDa.
Epitope:
GSK3B interaction domain
Immunogen:
KLH-conjugated linear peptide corresponding to the GSK3B interaction domain of mouse Dynamin related protein 1 (Drp1).
Background Information:
Dynamin related protein 1 (Drp1), also known as Dynamin-like protein 1, mediates outter mitochondrial membrane fission in mammilian cells. Like other members of the Dynamin superfamily of GTPases, Drp1 contains a N-terminal tripartate GTP-binding domain and exists as a T-shaped dimer with a head, leg, and stalk. The addition of GTP induces head and stalk rearrangement resulting in membrane constriction. Drp1 has been shown to affect mitochondrial morphology and places a significant role in mitochondrial and peroxisomal fission in mammilian cells. During apoptosis, Drp1 translocates from the cytosol to mitochondria and localizes to potential organelle division sites. Studies show Drp1 inhibition averts cell death, reinforcing to the critical role that mitochondrial fission plays in apoptosis.
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Species Reactivity:
Human
Mouse
Rat
Ox
Rhesus Macaque
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Species Reactivity Note:
Demonstrated to react with Human and Mouse.
Predicted to react with Rat, Ox, and Rhesus Macaque based on 100% sequence homology.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Dynamin related protein 1 in human embryonic stem cell H9 cells.
Control:
Mouse embryonic stem cell lysate
Quality Assurance:
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin related protein 1 (Drp1) on 10 µg of mouse embryonic stem cell lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q8K1M6
Entrez Gene Number:
NP_690029
Gene Symbol:
Dnm1l
Drp1
Alternate Names:
Dynamin-1-like protein
Dynamin family member proline-rich carboxyl-terminal domain less
Dymple
Dynamin-related protein 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
UniProt Summary:
FUNCTION: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around th |