|
Anti-Syndecan-4
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Syndecan-4 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes the cytoplasmic domain of Syndecan-4
Molecular Weight:
~80/32 kDa observed
Epitope:
Cytoplasmic domain
Immunogen:
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Syndecan-4.
Background Information:
Syndecan-4 (Amphiglycan; Ryudocan core protein) is one of four members of the syndecan proteoglycan family. The syndecan proteins bind heparan sulfate and mediate the signaling of growth factors, such as fibroblast growth factor, that recognize and bind heparan chains. Unlike other members of this family, syndecan-4 is ubiquitously expressed, and is targeted to focal adhesions, possibly by a PKC-mediated pathway. Previous studies have reported an increase in focal adhesions in cells overexpressing syndecan-4. This protein also plays an important role in wound healing, by modulating the signaling of Rho-GTPases Rac1, RhoG, and RhoA.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Human, Mouse, and Rat. Predicted to react with Bovine, Chimpanzee, and Rhesus Macaque based on 100% sequence homology.
Application Notes:
Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected Syndecan-4 in human lung carcinoma and in distal tubules of normal human kidney tissue.
Control:
HEP2 cell lysate
Quality Assurance:
Evaluated by Western Blot in HEP2 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Syndecan-4 in 10 µg of HEP2 cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P31431
Entrez Gene Number:
NP_002990
Gene Symbol:
SDC4
Alternate Names:
SYND4
Amphiglycan
Ryudocan core protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Product Name:
Anti-Syndecan-4
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
This Anti-Syndecan-4 Antibody is validated for use in Western Blotting, IHC(P) for the detection of Syndecan-4. |