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Anti-Ubiquitin Antibody
Description:
Anti-Ubiquitin Antibody
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Specificity:
This antibody recognizes Ubiquitin at the N-terminus
Molecular Weight:
~8 kDa observed. The calculated molecular weight of this protein is 8 kDa, but has been observed at ~24 kDa due to tetramers. (Kvint, K., et al. (2008). Mol Cell.30(4):498-506.)
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminus of human Ubiquitin.
Background Information:
Ubiquitin functions as a marker to indicate the fate of other proteins in the cell. It can be attached as a single molecule or as a polyubiquitin chain. Although ubiquitinated proteins are oftentimes tagged for degradation by the 20S proteosome, ubiquitination can also indicate alternative fates depending on the lysine residue that is involved in linking the ubiquitin chain to the target protein.Ubiquitin chains linked by Lys33 to kinase substrates such as AMP-activated protein kinases, (AMPKα1, BRSK1, MARK3, MARK4, and NUAK1), may activate or deactivate these proteins, supporting the role for uniquitin in regulating signaling.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Human, Mouse, and Rat. Broad species reactivity expected based on sequence similarity.
Application Notes:
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Ubiquitin on 10 µg of HeLa cell lysate.
Control:
Serum starved HeLa cell lysate
Quality Assurance:
Evaluated by Immunoprecipitation in serum starved HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg of this antibody detected Ubiquitin on 5 µL of serum starved HeLa cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P0CG48
Entrez Gene Number:
NP_066289
Gene Symbol:
UBC
Alternate Names:
Polyubiquitin-C
Ubiquitin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Product Name:
Anti-Ubiquitin Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Rabbit |