ODN INH-47 Toll-Like Receptor 9 (TLR9) detects unmethylated CpG dinucleotides in bacterial or viral DNA inducing strong immunostimulatory effects. TLR9 activation can be mimicked by synthetic phosphorothioate-stabilized oligodeoxynucleotides (ODN) containing immune stimulatory “CpG motifs”. Recent studies suggest the existence of DNA sequences that can neutralize the stimulatory effect of CpG ODNs. A new class of inhibitory ODNs, named the class R (‘restritcted’) inhibitory ODNs has been described [1-2]. This class of inhibitory ODNs includes ODN INH-1, a potent inhibitor of TLR9-induced B cells and macrophages [3]. ODN INH-47 is a negative control for ODN INH-1. ODN INH-47 is a palindromic variant of ODN INH-1 in which the CCT and GGG have been replaced by random nucleotide triplets. ODN INH-47 does not inhibit TLR9-induced activity in B cells or macrophages [3]. Working concentration: 100 nM - 10 µM Solubility: 5 mg/ml in water ODN INH-47 sequence 5’-tatggattttaa:ttaaaatccata-3’ Note: Bases are phosphorothioate (nuclease resistant). Quality control - The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.