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ODN INH-1
Toll-Like Receptor 9 (TLR9) detects unmethylated CpG dinucleotides in bacterial or viral DNA inducing strong immunostimulatory effects. TLR9 activation can be mimicked by synthetic phosphorothioate-stabilized oligodeoxynucleotides (ODN) containing immune stimulatory “CpG motifs”. Recent studies suggest the existence of DNA sequences that can neutralize the stimulatory effect of CpG ODNs.
ODN INH-1 belongs to a new class of inhibitory ODNs [1]. It contains an inhibitory DNA motif consisting of two nucleotide triplets, a proximal CCT and a more distal GGG, spaced from each other by four nucleotides. ODN INH-1 derives from ODN 4084-F by addition of a complementary strand of nucleotides forming a complete palindrome. ODN INH-1 is palindromic and a class R (‘restricted’) inhibitory ODN. ODN INH-1 is a potent inhibitor of TLR9-induced B cells and macrophages [2].
Specificity: Class R inhibitory ODN
Working concentration: 100 nM - 10 µM
Solubility: 5 mg/ml in water
ODN INH-1 sequence
5’-cctggatgggaa:ttcccatccagg-3’
Note: Bases are phosphorothioate (nuclease resistant).
Quality control
- The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells. |