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Anti-mTOR, clone F11
Species Reactivity Key Applications Host Format Antibody Type
H, R WB, IP Mouse Purified Monoclonal Antibody
Description:
Anti-mTOR Antibody, clone F11
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Molecular Weight:
~ 289 kDa observed
Immunogen:
GST-tagged recombinant protein corresponding to rat mTOR.
Clone:
F11
Isotype:
IgG1κ
Background Information:
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with rat based on 100% sequence homology.
Application Notes:
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Nemazanyy, I, et al. (2008). Hybridoma, 27:395-398.)
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on 10 µg of HeLa cell lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P42346
Entrez Gene Number:
NP_063971
Gene Symbol:
FRAP
FRAP1
FRAP2
RAFT1
RAPT1
mTOR
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Alternate Names:
FK506 binding protein 12-rapamycin associated protein 1
FK506 binding protein 12-rapamycin associated protein 2
FK506-binding protein 12-rapamycin complex-associated protein 1
FKBP-rapamycin associated protein
FKBP12-rapamycin complex-associated protein 1
Mammalian target of rapamycin
Rapamycin target protein
mechanistic target of rapamycin (serine/threonine kinase)
rapamycin and FKBP12 target 1
rapamycin associated protein FRAP2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Entrez Gene Summary:
Binds the complex formed by the immunosuppressive drug rapamycin and its receptor FKBP12; may play a role in the cell cycle G1 to S transition [RGD].
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