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Anti-phospho IRS1 (Ser318), clone 3.1.1
Species Reactivity Key Applications Host Format Antibody Type
H WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho IRS1 (Ser318) Antibody, clone 3.1.1
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Specificity:
This antibody recognizes IRS1 when phosphorylated at Ser318.
Molecular Weight:
~160 kDa observed. This protein has several post-translational modifications; therefore, the molecular weight will vary between ~150-170 kDa.
Epitope:
Phosphorylated Ser318
Immunogen:
KLH-conjugated linear peptide corresponding to mouse IRS1 when phosphorylated at Ser318.
Clone:
3.1.1
Isotype:
IgG1κ
Background Information:
IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Control:
Calyculin/okadaic acid treated and untreated CHO IR/IRS1 cell lysates
Quality Assurance:
Evaluated by Western Blot in calyculin/okadaic acid treated and untreated CHO IR/IRS1 cell lysates.
Western Blot Analysis: 0.5 µg/mL of this antibody detected IRS1 on 10 µg of calyculin/okadaic acid untreated and treated CHO IR/IRS1 cell lysates.
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Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P35569
Entrez Gene Number:
NP_034700
Gene Symbol:
HIRS-1
IRS-1
Alternate Names:
insulin receptor substrate 1
IRS
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
SUBUNIT STRUCTURE: Interacts (via phosphorylated YXXM motifs) with PIK3R1 (By similarity). Interacts with ROCK1 (By similarity). Interacts with GRB2 (By similarity). Interacts with SOCS7 (By similarity). Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif |