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Anti-GRK6, clone 4B11.2
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Mouse Purified Monoclonal Antibody
Description:
Anti-GRK6 Antibody, clone 4B11.2
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Molecular Weight:
~75 kDa observed. 66 kDa calculated.
Immunogen:
Histidine-tagged recombinant protein corresponding to human GRK6.
Clone:
4B11.2
Isotype:
IgG2aκ
Background Information:
G protein-coupled receptor kinase 6 (GRK6) is a widely expressed member of the AGC Ser/Thr protein kinase family and is comprised of an AGC-kinase C-terminal domain, a protein kinase domain, and a RGS domain. GRK6, like other GRKs, possesses unique activity against activated G protein-coupled receptors, which serves to regulate activation-dependent desensitization of the GPCR active forms. Studies have shown a critical role for GRK6 in the regulation of lymphocyte CXCR4 activity.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with human, mouse, and rat.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected GRK6 on 10 µg of HeLa cell lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P43250
Entrez Gene Number:
NP_001004105
Gene Symbol:
GRK6
GPRK6
Alternate Names:
G protein-coupled receptor kinase 6
G protein-coupled receptor kinase GRK6
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq].
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Specifically phosphorylates the activated forms of G protein-coupled receptors.
CATALYTIC ACTIVITY: ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor.
TISSUE SPECIFICTY: Widely expressed.
PTM: It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
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Product Name:
Anti-GRK6, clone 4B11.2
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse
Applications:
Detect GRK6 |