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Anti-NEDD8-conjugating enzyme Ubc12, clone 9H1.1
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REFERENCES
Semen alloantigens and lymphocytotoxic antibodies in AIDS and ICL.
R S Root-Bernstein,S H DeWitt (1995) Genetica.95
AIDS dementia complex complicated by cytomegalovirus encephalopathy.
M Fiala,E J Singer,M C Graves,W W Tourtellotte,J A Stewart,C A Schable,R H Rhodes,H V Vinters (1993) Journal of neurology.240
Species Reactivity Key Applications Host Format Antibody Type
M, H WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-NEDD8-conjugating enzyme Ubc12 Antibody, clone 9H1.1
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Molecular Weight:
~ 21 kDa observed
Immunogen:
GST-tagged recombinant protein corresponding to human NEDD8-conjugating enzyme Ubc12.
Clone:
9H1.1
Isotype:
IgG1κ
Background Information:
NEDD8-conjugating enzyme Ubc12, an E2 enzyme necessary for NEDDylation, has been shown to form a thioester linkage with the ubiquitin like protein NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. The NEDD8 pathway is essential for cell cycle progression and morphogenesis.
Species Reactivity:
Mouse
Human
Species Reactivity Note:
Demonstrated to react with mouse and human.
Application Notes:
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected NEDD8-conjugating enzyme Ubc12 in NIH/3T3, A431, and HeLa cells.
Control:
NIH/3T3 cell lysate
Quality Assurance:
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NEDD8-conjugating enzyme Ubc12 on 10 µg of NIH/3T3 cell lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P61081
Entrez Gene Number:
NP_003960
Gene Symbol:
UBE2M
UBC12
Alternate Names:
neural precursor cell expressed
NEDD8 carrier protein
NEDD8 protein ligase
Ubiquitin-conjugating enzyme E2 M
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq].
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UniProt Summary:
FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
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