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Anti-Neuregulin-1, clone D11
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REFERENCES
Neuregulin-1 modulates the differentiation of neural stem cells in vitro through an interaction with the Swi/Snf complex.
Pirotte, D, et al. (2010) Mol. Cell. Neurosci., 43: 72-80 (2010)
Species Reactivity Key Applications Host Format Antibody Type
M WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-Neuregulin-1 Antibody, clone D11
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Specificity:
This antibody recognizes Neuregulin-1 at the extracellular domain.
Molecular Weight:
~29 kDa observed. This target has been shown to undergo differential splicing during development into ~30 different isoforms.
Epitope:
Extracellular domain
Immunogen:
His-tagged recombinant protein corresponding to mouse Neuregulin-1 at the extracellular domain.
Clone:
D11
Isotype:
IgG1κ
Background Information:
The group of Neuregulin-1 proteins are cell-cell signaling molecules and are ligands for receptor tyrosine kinases of the ErbB/HER subfamily. Type III NRG1 proteins (also referred to as CRD-NRG1 proteins) play a critical role in neural development, including myelination, motor and sensory neuron survival, and neuromuscular synapse development (Falls, 2003). Defects in animals lacking type III NRGs include retraction of nerve terminals from newly formed synapses, absence of Schwann cells from peripheral nerves, and loss of motor and sensory neurons (Wolpowitz et al., 2000). Available evidence indicates that most Type III NRG1 proteins are synthesized as transmembrane proteins with 2 TM domains, and that proteolytic processing produces a bioactive transmembrane form (NTFm) which serves as a juxtacrine signal (Wang, 2001; Falls, 2003).
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Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with mouse.
Application Notes:
Immunocytochemistry Analysis: A previous lot of this antibody was used successfully by an independent laboratory in IC. (Pirotte, D., et al.)
Control:
Mouse brain microsomal tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain microsomal tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected Neuregulin-1 on 10 µg of mouse brain microsomal tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q6DR99
Entrez Gene Number:
NP_848706
Gene Symbol:
GGF
NDF
GGF2
HGL
MST131
HRGA
HRG
HRG1
SMDF
ARIA
pro-NRG1
MSTP131
Pro-NRG1
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Alternate Names:
neuregulin 1
sensory and motor neuron derived factor
neu differentiation factor
pro-neuregulin-1, membrane-bound isoform
heregulin, alpha (45kD, ERBB2 p185-activator)
heregulin, alpha (45kD, ERBB2 p185-activator)
glial growth factor
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
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