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Anti-Neuroligin-1, clone N97A/31
Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Neuroligin-1 Antibody, clone N97A/31
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Molecular Weight:
~ 120 kDa observed. Other isoforms may be observed in some lysates.
Immunogen:
Recombinant protein corresponding to rat Neuroligin-1.
Clone:
N97A/31
Isotype:
IgG1κ
Background Information:
Neuroligin-1 is a neuronal cell surface protein belonging to the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses for their normal, functional development, and is necessary to the regulation of synaptic plasticity and the development of long-term memory within the adult amygdala in mammals. It is believed to participate in cell-cell-interaction through the assembly of intracellular junction by the binding of beta-neurexins, and may also be a factor in the maintenance and assembly of synaptic junctions. It is also thought to have involvement in excitatory synaptic specification. Within brain tissue, Neuroligin-1 is primarily observed in neurons and spinal cord.
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Species Reactivity:
Rat
Species Reactivity Note:
Demonstrated to react with rat.
Application Notes:
Immunohistochemistry Analysis: 1:300 dilution of this antibody from a previous lot detected Neuroligin-1 in rat adult brain tissue.
Control:
Rat brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Neuroligin-1 on 10 µg of rat brain membrane tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q62765
Entrez Gene Number:
NP_446320
Gene Symbol:
NLGN1
NL1
Alternate Names:
neuroligin 1
neuroligin-1, NLGN1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry
Western Blotting
Entrez Gene Summary:
transmembrane ligand for neurexins; involved in development and maturation of synaptic connections [RGD].
UniProt Summary:
FUNCTION: Neuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. Seems to play role in formation or maintenance of synaptic junctions. In vitro, triggers the de novo formation of presynaptic structures (By similarity). May be involved in specification of excitatory synapses.
SUBUNIT STRUCTURE: Interacts with neurexin 1-beta isoform 3/Beta 4B5A and isoform 4/Beta 4B5B. Interacts with neurexin 2-beta isoform 3/Beta 4B5A, isoform 4/Beta 4B5B, isoform 7/Beta 4B5A6 and issoform 8/Beta 4B5B6. Interacts with corresponding neurexin 3-beta isoforms. Interacts through its C-terminus with DLG4/PSD-95 third PDZ domain (By similarity). Interacts with AIP1 and PDZRN3.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Cell junction |