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Anti-Ataxin-1, 11NQ, clone N76/8
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REFERENCES
Protein aggregates are recruited to aggresome by histone deacetylase 6 via unanchored ubiquitin C termini.
Hui Ouyang,Yousuf O Ali,Mani Ravichandran,Aiping Dong,Wei Qiu,Farrell Mackenzie,Sirano Dhe-Paganon,Cheryl H Arrowsmith,R Grace Zhai (2012) The Journal of biological chemistry.287
Species Reactivity Key Applications Host Format Antibody Type
R, M IHC Mouse Purified Monoclonal Antibody
Description:
Anti-Ataxin-1 Antibody, 11NQ, clone N76/8
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Molecular Weight:
~ 85 kDa observed
Immunogen:
Linear peptide corresponding to mouse Ataxin-1.
Clone:
N76/8
Isotype:
IgG2bκ
Background Information:
Ataxin-1 is a member of the ATXN1 protein family and contains a single AXH domain. It is a neurodegenerative disorder protein thought to have a role in the metabolism of RNA as it has been shown to localize to the RNA and transcription dependent inclusions within the nucleus. A mutation of Ataxin-1 is the cause of spinocerebellar ataxia type-1 (SCA1), a progressive, neurodegenerative disease that is autosomal dominant and primarily affects the Purjinke cells found in brain stem neuronal populations and the cerebellum. Expression of Ataxin-1 is almost ubiquitous, except in the brain where it is isolated to populations of neurons.
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Species Reactivity:
Rat
Mouse
Species Reactivity Note:
Demonstrated to react with rat. Predicted to react with mouse based on immunogen design.
Application Notes:
Western Blot Analysis: A previous lot was used by an independent laboratory in rat brain tissue lysate. (James Trimmer, UC Davis/NIH NeuroMab Facility)
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Ataxin-1 in rat cerebral cortex tissue.
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Control:
Rat hippocampus tissue
Quality Assurance:
Evaluated by Immunohistochemistry in rat hippocampus tissue.
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Ataxin-1 in rat hippocampus tissue.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P54254
Entrez Gene Number:
NP_033150
Gene Symbol:
ATXN1
ATX1
SCA1
Alternate Names:
ataxin 1
Spinocerebellar ataxia type 1 protein
spinocerebellar ataxia 1 (olivopontocerebellar ataxia 1, autosomal dominant, ataxin 1)
ataxin-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry
UniProt Summary:
FUNCTION: May be involved in RNA metabolism.
SUBUNIT STRUCTURE: Homooligomer By similarity. Interacts with PQBP1, UBIN and USP7 By similarity. Interacts with ANP32A. Interacts with CIC and ATXN1L. Interacts with ZNF804A (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus. Note: Colocalizes with USP7 in the nucleus (By similarity).
TISSUE SPECIFICTY: Widely expressed. In brain, the pattern of distribution is limited to neuron populations.
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