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Anti-pan-Shank, clone N23B/49
Species Reactivity Key Applications Host Format Antibody Type
R, M, H WB, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-pan-Shank Antibody, clone N23B/49
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Molecular Weight:
~160 kDa observed. Other isoforms may be observed in some lysates.
Immunogen:
Recombinant protein consisting of SH3/PDZ domain of rat Shank2.
Clone:
N23B/49
Isotype:
IgG1κ
Background Information:
Shank1, Shank2, and Shank3 make up a family of proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank contains multiple domains for protein-protein interaction including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile α motif domain, and a proline-rich region. Alternative splicing in the Shank family may be a mechanism that regulates the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain.
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Species Reactivity:
Rat
Mouse
Human
Species Reactivity Note:
Demonstrated to react with human, mouse, and rat.
Application Notes:
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Shank in rat cerebellum tissue.
Western Blot Analysis: A previous lot of this antibody detected Shank in extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids. Courtesy of James Trimmer, UC Davis/NIH NeuroMab Facility.
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Control:
Rat brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Shank on 10 µg of rat brain membrane tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9QX74
Entrez Gene Number:
NP_001004133
Gene Symbol:
SHANK2
CORTBP1
ProSAP1
CTTNBP1
SPANK-3
SHANK
PROSAP1
CortBP1
Shank2
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Alternate Names:
SH3 and multiple ankyrin repeat domains 2
SH3 and multiple ankyrin repeat domains protein 2
proline-rich synapse associated protein 1
cortactin SH3 domain-binding protein
Cortactin-binding protein 1
Proline-rich synapse-associated protein 1
cortactin binding protein 1
GKAP/SAPAP interacting protein
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
SUBUNIT STRUCTURE: Interacts with DNM2 and BAIAP2. Interacts with GRID2 (By similarity). Intera |