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Anti-Beclin-1 (C-term), clone EPR1733Y, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H IH(P), IP, ICC, WB Rabbit Unpurified Monoclonal Antibody
Description:
Anti-Beclin-1 (CT) Antibody, clone EPR1733Y, rabbit monoclonal
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Specificity:
This antibody recognizes Beclin-1 near the C-terminus.
Molecular Weight:
~52 kDa observed
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to human Beclin-1 near the C-terminus.
Clone:
EPR1733Y
Isotype:
IgG
Background Information:
The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human.
Application Notes:
Immunohistochemistry Analysis: A 1:50 dilution from a previous lot detected Beclin-1 in human breast tissue.
Immunocytochemistry Analysis: A 1:50 – 1:100 dilution from a previous lot detected Beclin-1 in HeLa cells.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot detected Beclin-1 in IP.
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Control:
A431 cell lysate
Quality Assurance:
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected Beclin-1 in A431 cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q14457
Entrez Gene Number:
NP_003757.1
Gene Symbol:
ATG6
VPS30
beclin1
beclin-1
GT197
Protein GT197
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Alternate Names:
beclin 1, autophagy related
beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
Coiled-coil myosin-like BCL2-interacting protein
ATG6 autophagy related 6 homolog
beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are i |