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Anti-Histone H3.3
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REFERENCES
Hira-Dependent Histone H3.3 Deposition Facilitates PRC2 Recruitment at Developmental Loci in ES Cells.
Banaszynski, Laura A, et al. (2013) Cell, 155: 107-20 (2013)
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC, DB, ChIP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Histone H3.3 Antibody
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Molecular Weight:
~17 kDa observed
Immunogen:
KLH-conjugated linear peptide corresponding to human Histone H3.3.
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Demonstrated to react with Human and Mouse. Broad species cross-reactivity expected based on 100% sequence homology.
Application Notes:
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Histone H3.3 in HeLa cells.
Image courtesy of Simon Elsässer of David Allis' Laboratory, Rockefeller University, New York.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP.
Image courtesy of Simon Elsässer of David Allis' Laboratory, Rockefeller University, New York.
Dot Blot Analysis: A representative lot was used by an independent laboratory in DB.
Reported by Simon Elsässer, Laboratory of Dr. David Allis, Rockefeller University, New York.
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Control:
HeLa acid extract
Quality Assurance:
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3.3 on 10 µg of HeLa acid extract.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P84243
Entrez Gene Number:
NP_002098
Gene Symbol:
H3F3A
H3.3A
H3F3
PP781
H3F3B
H3.3B
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Alternate Names:
Histone H3.3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Dot Blot
Chromatin Immunoprecipitation (ChIP)
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp |