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Anti-KLF5 (Krüppel-like factor 5)
Species Reactivity Key Applications Host Format Antibody Type
M WB, ChIP Rabbit Serum Polyclonal Antibody
Description:
Anti-KLF5 (Krüppel-like factor 5) Antibody
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Specificity:
This antibody recognizes Krüppel-like factor 5 at the N-terminus.
Molecular Weight:
~55 kDa observed. An uncharacterized band appears at ~30 kDa.
Epitope:
N-terminus
Immunogen:
Recombinant protein corresponding to mouse Krüppel-like factor 5 at the N-terminus.
Background Information:
Krüppel-like factors (KLFs) are zinc finger-containing transcription factors with regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. KLF5, (also known as BTEB2 and IKLF), is essential for mouse embryo development. KLF5 activates many genes during cardiovascular remodeling, including PDGFa/b, Egr-1, PAI-1, iNOS, and VEGF receptors, making KLF a potential therapeutic target for cardiovascular disease. KLF5 also plays a role in angiogenesis and in development of several tumor types including breast, prostate, bladder, colon, esophagus, and skin. KLF5 is closely related to other of the KLF family members. However, KLF5 and KLF4 exhibit opposite effects on regulation of gene transcription, despite binding to similar, if not identical, cis-acting DNA sequences. In addition, KLF4 and 5 exert contrasting effects on cell proliferation in many instances; while KLF4 is an inhibitor of cell growth, KLF5 stimulates proliferation.
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Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with mouse.
Application Notes:
Chromatin Immunoprecipitation Analysis (ChIP):
A representative lot was used by an independent laboratory in ChIP. (Ng Huck Hui, Genome Institute of Singapore)
Control:
Mouse embryonic stem cell chromatin extract
Quality Assurance:
Evaluated by Western Blot in mouse embryonic stem cell chromatin extract.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Krüppel-like factor 5 on 10 µg of mouse embryonic stem cell chromatin extract.
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Purification Method:
Unpurified
Presentation:
Rabbit polyclonal serum containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q9Z0Z7
Entrez Gene Number:
NP_033899
Gene Symbol:
KLF5
CKLF
BTEB2
IKLF
Alternate Names:
BTE-binding protein 2
Basic transcription element-binding protein 2
Colon krueppel-like factor
GC box binding protein 2
GC-box-binding protein 2
Intestinal-enriched krueppel-like factor
Kruppel-like factor 5
Kruppel-like factor 5 (intestinal)
Transcription factor BTEB2
basic transcription element binding protein 2
colon kruppel-like factor
intestinal-enriched kruppel-like factor
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animal |