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Anti-Krüeppel-like factor 4 (Klf4) Antibody (09-821)
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Species Reactivity Key Applications Host Format Antibody Type
M, H WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-KLF4 Antibody
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Specificity:
This antibody recognizes Klf4 at the N-terminus.
Molecular Weight:
~55 kDa observed
Epitope:
N-terminus
Immunogen:
GST-tagged recombinant protein corresponding to the N-terminus of mouse Klf4.
Background Information:
Krüppel-like factors (KLFs) are zinc finger-containing transcription factors with regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. Krüppel-like factor 4 (Klf4) has diverse functionss and acts as both an activator and repressor of transcription. The Klf protein has been shown to activate its own transcription throught its interaction with CACCC core sequences. High expression of Klf4 is observed in differentiating layers of epidermis and a loss of expression results in a break-down of barrier function including protection against pathogen invasion and dehydration. KLF4 is also associated with other biological functions such as proliferation, differentiation and carcinogenesis in gastrointestinal tissues. After DNA damage, Klf4 has been shown to induce DNA repair pathways. Klf4 seems to hinder prostate cancer tumor cell growth and migration.
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Species Reactivity:
Mouse
Human
Species Reactivity Note:
Demonstrated to react with mouse and human.
Application Notes:
Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected Klf4 in NIH/3T3, A431, and HeLa cells.
Control:
Mouse embryonic stem cell chromatin extract
Quality Assurance:
Evaluated by Western Blot in mouse embryonic stem cell chromatin extract.
Western Blot Analysis: 1:500 dilution of this antibody detected Klf4 on 10 µg of mouse embryonic stem cell chromatin extract.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q60793
Entrez Gene Number:
NP_034767.2
Gene Symbol:
KLF4
EZF
GKLF
Alternate Names:
Kruppel-like factor 4 (gut)
Gut-enriched krueppel-like factor
endothelial Kruppel-like zinc finger protein
Krueppel-like factor 4
Epithelial zinc finger protein EZF
Krüppel-like factor
klf
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
UniProt Summary:
FUNCTION: Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal |