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FlowCellect™ Bcl-2 Activation Dual Detection Kit
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PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect™ Bcl-2 Activation Dual Detection Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Bcl-2 Activation Dual Detection kit includes two directly conjugated antibodies: Anti-Bcl-2-Alexa Fluor® 488 and Anti-pBcl-2 (Serine 70)-PE, along with optimized fixation, permeabilization, wash, and assay buffers necessary for cell preparation and analysis. Millipore’s FlowCellect™ Activation Dual Detection Kit contains sufficient reagents for 25 2-color samples. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
Millipore’s FlowCellect™ Bcl-2 Activation Dual Detection Kit is designed to enable a researcher a quick and easy way to detect total Bcl-2 expression as well as the phosphorylation of Bcl-2 at serine 70 simultaneously. Millipore’s FlowCellect™ Activation Dual Dectection Kit was developed and tested using Jurkat cells treated with staurosporine, but the kit can be used with other human cell lines to determine the effect of other chemical reagents on Bcl-2 expression and phosphorylation.
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Background Information:
The signaling pathways that regulate apoptosis are important in understanding tissue homeostasis, the development of the immune system, and therapies for cancer. Bcl-2 is a member of the Bcl-2 family that regulates apoptosis by regulating the proteins that control mitochondrial membrane permeability.
The Bcl-2 family consists of two protein sub-groups, the anti-apoptotic proteins and the pro-apototic proteins (3). Bcl-2 is an anti-apoptotic family member that binds to pro-apoptotic family members, such as Bax to sequester them and inhibit insertion into the mitochondrial membrane. When cells receive an apoptotic signal, Bcl-2 releases Bax, allowing Bax to form a complex on the mitochondrial membrane that releases cytochrome C into the cytoplasm leading to caspase activation and cell death.
Bcl-2 has been shown to be regulated at the level of phosphorylation by JNK at a multi-site phosphorylation loop which includes serine 70. Phosphorylation of Bcl-2 at Serine 70 has been shown to be important in both apoptosis and in autophagy.
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Applications:
This FlowCellect Bcl-2 Activation Dual Detection Kit for Flow Cytometry includes two directly conjugated antibodies: Anti-Bcl-2-Alexa Fluor 488 & Anti-pBcl-2 (Serine 70)-PE.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Alexa Fl |