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FlowCellect™ EGFR RTK Activation Dual Detection Kit
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PRODUCT FAMILY INFORMATION
EGFR Antibodies, Kits & Proteins
Millipore’s EGFR (Epidermal growth factor receptor) antibodies, kits, and proteins are high quality, validated tools for EGFR research. See below for a selection of EGFR and phospho-EGFR products.
Millipore’s EGFR antibodies, kits and proteins are high quality, validated tools for EGFR research. See below for data and references for Millipore’s EGFR products. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect™ EGFR RTK Activation Dual Detection Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
EGF pathway activation through EGFR plays a key role in the regulation of essential cellular processes, and abnormality of EGF signaling is linked to cancer.
Millipore’s FlowCellect™ EGFR RTK Activation Dual Detection kit includes two directly conjugated antibodies, a phospho-specific Anti-phospho-EGFR (Tyr1173)-Alexa Fluor® 488 and an Anti-EGFR-PerCP conjugated antibody to measure total levels of EGFR. This two color flow cytometry kit is designed to detect the extent of EGF pathway activation by measuring the EGFR phosphorylation in relative to the total EGFR expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of EGFR activation after stimulation. Moreover, simultaneous measurement of both total and phospho-EGFR confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho:total ratio within a mixed population.
All FlowCellect kits are supplied with optimized fixation, permeabilization, wash, and assay buffers to provide researchers the ability to detect the activation of EGFR in flow applications. Sufficient reagents are provided for 25 two-color samples. The kit includes all optimized fluorescently labeled antibodies and buffers necessary for cell preparation and analysis.
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Background Information:
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. EGFR is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
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