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FlowCellect™ p38 Stress Pathway Activation Detection Kit
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PRODUCT FAMILY INFORMATION
MAPK Antibodies, Proteins, Inhibitors, & Assays
Offering hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research
EMD Millipore offers hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research. All EMD Millipore products are based on the expertise of Upstate, Chemicon, and Calbiochem.
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Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect™ p38 Stress Pathway Activation Detection Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ p38 Stress Pathway Activation Detection Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of P38α and ATF2 by flow cytometry. Millipore’s FlowCellect™ p38 Stress Pathway Activation Kit was developed and tested using the activating reagent, anisomycin, in Jurkat cells but the kit can be used with other human cell lines to determine the effect of stress stimuli and chemical reagents that can induce P38 pathway signaling.
Millipore’s FlowCellect™ p38 Stress Pathway Activation Detection Kit contains sufficient reagents for 25 2-color tests. The kit includes two optimized fluorescent labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
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Background Information:
p38 is a member of the mitogen activated kinase family that responds to stress stimuli, such as inflammatory cytokines, ultraviolet irradiation, heat shock, and osmotic shock. p38 is involved in cell differentiation and apoptosis. More recently, P38 has been shown to have a role in cell cycle check points and DNA damage pathways. The upstream kinases, MKK3, MKK6 and SEK activate p38α by phosphorylation at Thr180 and Tyr182.
Once activated, p38α MAP kinase has been shown to phosphorylate and activate a number of proteins including MAPKAP kinase 2 and the transcription factors ATF-2, MEF2 and MSK1.
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Applications:
This FlowCellect p38 Stress Pathway Activation Detection Kit for Flow Cytometry is designed to enable a researcher a quick & easy way to detect the phosphorylation state of P38α & ATF2 by flow cytometry.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
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