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FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis
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PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis is designed to allow the researcher to easily identify, discriminate, and quantitate cells between the G2 and M-phases of the cell cycle. The kit can be used to perform bivariate analysis to measure cell cycle distributions by discrimination of cell cycle phases without the need of sophisticated software modules or algorithms. Having the ability to distinguish cells undergoing mitosis versus cells remaining in G2 phase of the cell cycle provides researchers with a powerful tool to closely monitor cell cycle activity at M-phase which can have a huge impact in studying diseases associated with cell proliferation, apoptosis, and cancer.
All FlowCellect kits are optimized on the bench-top guava® flow cytometry systems, which saves valuable time and sample volume. All cell cycle kits contain optimized fixation, permeabilization, wash, and flow buffers to provide researchers with a complete solution for bivariate analysis of total DNA content versus cell cycle phase markers. With the guava platform and FlowCellect kits, researchers can finally have an easy, reliable and fully validated solution to study complex cell cycle distribution analysis right in the comfort of their own lab.
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Background Information:
Examination of the cell cycle has proven to be an accurate and effective method for assessing cell health by analyzing cell proliferation in great detail. Also recognized as the “Circle of Life”, the cell cycle can be divided into two distinct stages. The first stage is Interphase which consists of the G1, S, and G2 phases where cells are active, growing, and DNA replication takes place. The second is M-phase, also known as the “mitotic phase”, where cell division takes place. Researchers have employed various strategies to closely interrogate each of the critical steps at various phases in the cell cycle. For example, analysis of cells in S-phase will provide a direct measurement of newly synthesized DNA. Also, having the ability to discriminate cells between the G2 and M-phases can serve as a good indicator of cells undergoing mitosis. In all, a comprehensive understanding of cell cycle behavior can provide the researcher with useful information which will be important in interpreting the intrinsic nature of cell proliferation and assist in the development of anti-neoplastic agents.
Flow cytometric analysis of cell DNA content is widely used for the estimation of cell cyc |