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FlowCellect™ Bivariate Cell Cycle Kit for DNA Replication Analysis
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PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect™ Bivariate Cell Cycle Kit for DNA Replication Analysis
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Bivariate Cell Cycle Kit for DNA Replication Analysis is designed to investigate cells undergoing replication in S-phase of the cell cycle with high accuracy and confidence in flow cytometry applications. The kit includes a directly conjugated Anti-BrdU Alexa Fluor® 488 conjugate plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to distinguish cells in two dimensions without the need of software modules for data interpretation. Flow cytometry allows the simultaneous measurement of incorporated BrdU as well as the DNA content on a single cell level. In this way, the cohort of labeled cells can be followed through the cell cycle.
The kit includes an optimized protocol with all of the necessary components to provide the researcher a true “plug and play” design for their research when studying DNA replication and its relation to cell proliferation and cancer. The kit includes a BrdU precursor to allow for BrdU measurement by the conjugated antibody and an RNase reagent to ensure that the PI intensity is directly proportional to DNA content only. This immunocytochemical method is very sensitive, and it offers an assay to screen in vivo inhibitors involved in the S-phase transition by flow cytometry. It is important to determine the biological characteristics of certain cancers, not only to evaluate their malignant potentials but also to decide on the most effective anticancer therapies.
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Background Information:
Examination of the cell cycle has proven to be an accurate and effective method for assessing cell health by analyzing cell proliferation in great detail. Also recognized as the “Circle of Life”, the cell cycle can be divided into two distinct stages. The first stage is Interphase which consists of the G1, S, and G2 phases where cells are active, growing, and DNA replication takes place. The second is M-phase, also known as the “mitotic phase”, where cell division takes place. Researchers have employed various strategies to closely interrogate each of the critical steps at various phases in the cell cycle. For example, analysis of cells in S-phase will provide a direct measurement of newly synthesized DNA. Also, having the ability to discriminate cells between the G2 and M-phases can serve as a good indicator of cells undergoing mitosis. In all, a comprehensive understanding of cell cycle behavior can provide the researcher with useful informa |