|
FlowCellect™ Multi-Color DNA Damage Response Kit
Close
PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
» More…
Description:
FlowCellect™ Multi-Color DNA Damage Response Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of ATM, SMC1 and Histone H2A.X by flow cytometry. Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit was developed and tested using the DNA damaging reagent, Etoposide, in HeLa cells as a model system but the kit can be used with other human cell lines to determine the effect of mechanical and chemical reagents that can induce DNA damage through the ATM dependent pathway.
Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit contains sufficient reagents for 25 3-color samples. The kit includes three optimized fluorescently labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
View All »
Background Information:
The signaling pathways by which cells respond to DNA damage is an important area of study for cancer research. The mammalian cell response to DNA damage is made up of highly coordinated signaling pathways that can initiate cell cycle arrest and repair or initiate apoptosis, depending on the extent and type of the damage. Cells that are defective in DNA damage signaling pathways can cause cancer because they lack the ability to sense and repair the damage, leading to genetic instability and ultimately uncontrolled cell growth.
The main kinase activated in response to double-stranded DNA breaks is ATM or Ataxia-telangiectasia mutated kinase. ATM is a member of the phospho inositide 3-kinase (PI3K)-related Ser/Thr protein kinase family. Inactive ATM exists as a dimer but quickly dissociates and becomes phosphorylated on Serine 1981 in response to ionizing radiation.
Once activated, ATM phosphorylates a number of downstream factors, including P53, CHK2, SMC1, NBS1 and Histone H2A.X. SMC1 is a member of the structural maintenance of chromosome family originally identified in yeast to be important for proper segregation of the chromosomes during mitosis. However, in mammalian cells exposed to IR, phosphorylation of SMC1 on sites S957 and S966 by ATM has been shown to be required for activation of the S-phase checkpoint and is important for protection against radiation induced cell death.
Phosphorylation of the histone variant, H2A.X, at serine 139 by ATM is an important indicator of DNA damage. As |