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Milli-Mark™ Anti-monomethyl-Histone H3 (Lys27)-Alexa Fluor® 488
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PRODUCT FAMILY INFORMATION
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Species Reactivity Key Applications Host Format Antibody Type
H FC Rabbit AlexaFluor®488 Polyclonal Antibody
Description:
Milli-Mark™ Anti-monomethyl-Histone H3 (Lys27)-Alexa Fluor® 488 Antibody
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Trade Name:
Milli-Mark
Specificity:
Antibody recognizes monomethylated histone H3 (Lys27)
Molecular Weight:
15.5 kDa Calculated
Immunogen:
KLH-conjugated, 2X-branched synthetic peptide corresponding to monomethyl-lysine at residue 27 of human Histone H3.
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
HeLa cells
Quality Assurance:
Evaluated by flow cytometry using HeLa cells
Purification Method:
Protein A Purified
Presentation:
Purified rabbit polyclonal IgG conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
UniProt Number:
Q16695
Entrez Gene Number:
NP_003484
Gene Symbol:
HIST3H3
H3FT
H3/t
H3t
MGC126888
H3/g
H3.4
H3T
MGC126886
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Alternate Names:
H3 histone fami |