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MMP-9 Immunoassay Kit, proform only
Description:
MMP-9 Immunoassay Kit, proform only
Trade Name:
Chemicon (Millipore)
Qty/Pk:
100 assays
Product Overview:
The MMP-9 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same MMP-9 molecule. Upon the addition of an MMP-9-containing specimen, the MMP-9 molecule is sandwiched between the antibody coated on the bead and enzyme-labeled antibodies. After removing unbound enzyme-labeled antibody, the bead containing the MMP-9 complex is incubated with enzyme substrate and o-phenylenediamine, resulting in the development of color. The resultant color enzyme is proportional to the amount of MMP-9 present, the concentration of MMP-9 in specimens can be determined from a standard curve.
Test Principle:
The MMP-9 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same MMP-9 molecule. Upon the addition of an MMP-9-containing specimen, the MMP-9 molecule is sandwiched between the antibody coated on the bead and enzyme-labeled antibodies. After removing unbound enzyme-labeled antibody, the bead containing the MMP-9 complex is incubated with enzyme substrate and o-phenylenediamine, resulting in the development of color. The resultant color enzyme is proportional to the amount of MMP-9 present, the concentration of MMP-9 in specimens can be determined from a standard curve.
Application:
The MMP-9 immunoassay kit is useful for the determination of MMP-9 (pro-MMP-9) levels in fresh human plasma and conditioned medium of human cells. Human serum is not an acceptable sample for evaluation.
This system recognizes free pro-MMP-9, intermediate 83 kDa MMP-9, and MMP-9 in complex with TIMP-1 with the same efficiency. The assay does not recognize active MMP-9 (67 kDa). Contents of this kit are sufficient for assay of 100 samples, including standard curve. Testing of samples in duplicate or triplicate is strongly recommended. This kit is intended for research use only; not for diagnostic or therapeutic applications.
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Applications:
The MMP-9 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same MMP-9 molecule.
Key Applications:
Activity Assay
Species Reactivity:
Human Only
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
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UniProt Summary:
FUNCTION: SwissProt: P22894 # Can degrade fibrillar type I, II, and III collagens.
COFACTOR: Binds 3 calcium ions per subunit. & Binds 2 zinc ions per subunit.
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