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MMP-2 Immunoassay Kit, pro-form only
Description:
MMP-2 Immunoassay Kit, pro-form only
Trade Name:
Chemicon (Millipore)
Qty/Pk:
100 assays
Product Overview:
Matrix metalloproteinase 2 (72 kDa gelatinase, gelatinase A, MMP-2) has been identified in a number of tissues, cells and blood. The precursor form of MMP-2 (pro-MMP-2) is tightly associated with tissue inhibitor of metalloproteinases-2 (TIMP-2) and regulated autoactivation.
Pro-MMP-2 may be quantitated using this sensitive immunoassay against
MMP-2
Test Principle:
The MMP-2 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same MMP-2 molecule. Upon addition of an MMP-2-containing specimen, the result is an MMP-2 molecule being sandwiched between the solid phase and enzyme labeled antibodies. After removing unbound enzyme-labeled antibody, the bead containing the sandwich is incubated with enzyme substrate and o-phenylenediamine, resulting in the development of color. The activity of peroxidase enzyme is proportional to the amount of antigen, MMP-2, so that MMP-2 concentration in specimens can be determined from a standard curve.
Application:
The MMP-2 immunoassay kit is useful for the determination of MMP-2 (pro-MMP-2) levels in fresh human sera.
The values measured by using this kit represent pro-MMP-2 concentrations, because pro-MMP-2 is used as a standard in this kit. This system recognizes both free pro-MMP-2 and pro-MMP-2 complexed with TIMP-2. It does not recognize active MMP-2. Contents of this kit are sufficient for assay of 100 samples, including standard curve. Testing of samples in duplicate or triplicate is strongly recommended. This kit is intended for research use only; not for diagnostic or therapeutic applications.
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Applications:
The MMP-2 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same MMP-2 molecule.
Key Applications:
ELISA
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome.
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UniProt Summary:
FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 660 amino acids; 73882 Da
SUBUNIT: Ligand for integrin alpha-V/beta-3.
TISSUE SPECIFICITY: Produced by normal skin fibroblasts.
DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the c |