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Anti-RING1, clone 4D6
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-RING1 Antibody, clone 4D6
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Specificity:
This antibody detects RING1.
Molecular Weight:
~42 kDa
Immunogen:
GST-tagged recombinant protein corresponding to RING1.
Clone:
4D6
Isotype:
IgG2aκ
Background Information:
RING1 belongs to the RING finger family, members of which are characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and mouse.
Application Notes:
Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used in immunocytochemistry on NIH/3T3 and A431 cells.
Control:
HEK293 cell lysate
Quality Assurance:
Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected RING1 in HEK293 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Q06587
Entrez Gene Number:
NP_002922.2
Gene Symbol:
EC 6.3.2.-
OTTHUMP00000029416
RING1A
RNF1
Alternate Names:
Polycomb complex protein RING1
ring finger protein 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq].
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UniProt Summary:
FUNCTION: Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, inc |