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Anti-PURA, clone 2B7
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Mouse Purified Monoclonal Antibody
Description:
Anti-PURA Antibody, clone 2B7
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Specificity:
This antibody detects PURA.
Molecular Weight:
~35-37 kDa
Immunogen:
GST-tagged recombinant protein corresponding to PURA.
Clone:
2B7
Isotype:
IgG2aκ
Background Information:
Purine-rich element binding protein A, also known as PURA, is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse and rat.
Control:
A549 cell lysate
Quality Assurance:
Evaluated by western blot on A549 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected PURA in A549 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Q00577
Entrez Gene Number:
NP_005850.1
Gene Symbol:
PUR-ALPHA
PUR1
PURALPHA
Alternate Names:
transcriptional activator protein PUR-alpha
purine-rich element binding protein A
Purine-rich single-stranded DNA-binding protein alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq].
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UniProt Summary:
FUNCTION: This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.
SUBUNIT STRUCTURE: Homodimer, heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1 By similarity.
SUBCELLULAR LOCATION: Nucleus.
SEQUENCE SIMILARITIES: Belongs to the PUR DNA-binding protein family.
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Product Name:
Anti-PURA, clone 2B7
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse
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