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Anti-acetyl Histone H4 (Lys5/8/12/16), clone 3HH4-4C10
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PRODUCT FAMILY INFORMATION
Histone H4 Antibodies and Proteins
Millipore’s Histone H4 antibodies demonstrate specificity against linker histone H4. See below for H4 Antibodies, ChipAb+ Primer sets, and peptides, based on the expertise of Upstate & Chemicon.
EMD Millipore’s Histone H4 antibody demonstrates specificity against linker histone H4. See below for data and references for EMD Millipore’s Histone H4 antibodies and proteins. All EMD Millipore antibodies and proteins are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC Mouse Ascites Monoclonal Antibody
Description:
Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10
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Specificity:
This antibody recognizes Histone H4.
Molecular Weight:
~ 13 kDa
Epitope:
Lys5/8/12/16
Immunogen:
Linear peptide corresponding to human Histone H4 acetylated at Lys5/8/12/16.
Clone:
3HH4-4C10
Isotype:
IgG1κ
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Posttranslational acetylation of the conserved core histone N-terminal tail domains is typically associated with gene activation.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
Application Notes:
Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.
Control:
HeLa acid extract lysate
Quality Assurance:
Evaluated by Western Blot in HeLa acid extract lysate.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.
Purification Method:
Unpurified
Presentation:
Unpurified mouse monoclonal IgG1κ preservative-free ascites.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P62805
Entrez Gene Number:
NP_778224
Gene Symbol:
HIST1H4A
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIST1H4B
H4FE
H4/M
H4/a
HIST1H4E
HIST4H4
HIST2H4A
H4FC
H4FI
H4/H
HIST1H4C
H4/G
HIST1H4K
H4FJ
H4FD
H4/I
H4/B
H4/D
H4/C
HIST1H4J
HIST1H4D
H4/E
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Alternate Names:
H4KAc
Histone H4 (acetyl)
H4 histone family, member N
H4 histone, family 2
histone 2, H4
histone 2, H4a
histone IV, family 2
histone cluster 2, H4a
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