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Anti-Trimethyl Histone H3 (Lys4), clone CMA304
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REFERENCES
Global mapping of H3K4me3 and H3K27me3 reveals specificity and plasticity in lineage fate determination of differentiating CD4+ T cells.
Wei, Gang, et al. (2009) Immunity, 30: 155-67 (2009)
Whole-genome mapping of histone H3 Lys4 and 27 trimethylations reveals distinct genomic compartments in human embryonic stem cells.
Zhao, Xiao Dong, et al. (2007) Cell Stem Cell, 1: 286-98 (2007)
The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
Kimura, Hiroshi, et al. (2008) Cell Struct. Funct., 33: 61-73 (2008)
The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
Kimura, Hiroshi, et al. (2008) Cell Struct. Funct., 33: 61-73 (2008)
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt ELISA, ICC, IP, WB, Mplex, DB Mouse Purified Monoclonal Antibody
Description:
Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304
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Specificity:
This antibody specifically recognizes Histone H3 trimethylated at Lys4. The antibody binding specificity allows for modifications of Lys91.
Molecular Weight:
approx. 17 kDa
Epitope:
Trimethylated Lys4
Immunogen:
Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, trimethylated on Lys4, conjugated to KLH.
Modifications:
Methylation
Clone:
CMA304
Isotype:
IgG1κ
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H3 (Lys4) is is found at the 5' end of active genes and contributes to transcriptional activation by recruiting chromatin-remodelling enzymes. Accordingly, the inactive X chromosome is largely devoid of this mark.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected, based on sequence homology.
Application Notes:
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
Multiplexing:
This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex® assay.
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Control:
HeLa Acid extract lysate
Quality Assurance:
Western Blot Analysis:
0.5 - 2 μg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to rem |