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Anti-Histone H3, (Unmodified Lys4), clone CMA301 Antibody (05-1341)
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REFERENCES
Regulation of nucleotide excision repair by UV-DDB: prioritization of damage recognition to internucleosomal DNA.
Fei, Jia, et al. (2011) PLoS Biol., 9: e1001183 (2011)
Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation.
Hendzel, M J, et al. (1997) Chromosoma, 106: 348-60 (1997)
Alteration of cell cycle-dependent histone phosphorylations by okadaic acid. Induction of mitosis-specific H3 phosphorylation and chromatin condensation in mammalian interphase cells.
Ajiro, K, et al. (1996) J. Biol. Chem., 271: 13197-201 (1996)
Increased phosphorylation of histone H1 in mouse fibroblasts transformed with oncogenes or constitutively active mitogen-activated protein kinase kinase.
Chadee, D N, et al. (1995) J. Biol. Chem., 270: 20098-105 (1995)
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt WB, ICC, ELISA, IP, FC, DB Mouse Affinity Purified Monoclonal Antibody
Description:
Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301
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Specificity:
This antibody recognizes Histone H3 that is unmodified at Thr3 and Lys4 but does not recognize phosphorylated Thr3 nor acetylated or methylated Lys4. The antibody binding specificity allows modifications of Lys9, Ser10, and/or Lys141.
Molecular Weight:
Approx. 17 kDa
Epitope:
N-terminus (unmodified)
Immunogen:
Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, conjugated to KLH.
Clone:
CMA301
Isotype:
IgG2bκ
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected, based on sequence homology.
Application Notes:
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
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Control:
HeLa Acid extract lysate.
Quality Assurance:
Routinely evaluated by Western Blot on HeLa cell lysate.
Western Blot Analysis: 1:10,000 dilution of this antibody detected histone H3 in 10 μg of HeLa Acid Extract cell lysate and recombinant histone H3 (500 ng) but not other recombinant histones.
Purification Method:
Peptide affinity chromatography
Presentation:
Mouse monoclonal IgG2bκ supernatant in PBS with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
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