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Anti-Dimethyl Histone H3 (Lys4), clone CMA303 Antibody
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REFERENCES
The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
Kimura, Hiroshi, et al. (2008) Cell Struct. Funct., 33: 61-73 (2008)
Differential H3K4 methylation identifies developmentally poised hematopoietic genes.
Orford, Keith, et al. (2008) Dev. Cell, 14: 798-809 (2008)
Inhibition of lysine-specific demethylase 1 by polyamine analogues results in reexpression of aberrantly silenced genes.
Huang, Yi, et al. (2007) Proc. Natl. Acad. Sci. U.S.A., 104: 8023-8 (2007)
DNA methylation dictates histone H3K4 methylation.
Okitsu, Cindy Yen and Hsieh, Chih-Lin (2007) Mol. Cell. Biol., 27: 2746-57 (2007)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt WB, IP, DB, ICC, ELISA, Mplex, ChIP Mouse Purified Monoclonal Antibody
Description:
Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303
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For the full resolution version of the ChIP-seq data Click Here.
Specificity:
This antibody specifically recognizes Histone H3 dimethylated at Lys4. The antibody binding specificity allows for phosphorylation of Thr3 and/or modifications of Lys91.
Molecular Weight:
approx. 17 kDa
Epitope:
Dimethylated Lys4
Immunogen:
Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, dimethylated on Lys4, conjugated to KLH.
Modifications:
Methylation
Clone:
CMA303
Isotype:
IgG1κ
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Dimethyl-lysine 4 histone H3 (H3K4me2) is a transcription-activating chromatin mark, and dimethyl histone H3 Lys4 (H3K4me2) is depleted from regions with DNA methylation. Multipotential hematopoietic cells have a subset of genes that are differentially methylated (H3K4me2+/me3-). These genes are transcriptionally silent and highly enriched in lineage-specific hematopoietic genes, suggesting a role for H3K4 methylation in differentiation.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected, based on sequence homology.
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG, or Anti-dimethyl-Histone H3 (Lys4) antibody and the Magna ChIP G (Cat. # 17-611) Kit. Successful immunoprecipitation of dimethyl-histone H3 (Lys4) associated DNA fragments was verified by qPCR using ChIP Primers GAPDH Coding.
Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Dot Blot Analysis:
Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat |