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Anti-phospho-Histone H3 (Ser10), clone CMA312
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PRODUCT FAMILY INFORMATION
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt WB, ELISA, ICC, Mplex Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312
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Specificity:
This antibody specifically recognizes Histone H3 phosphorylated at Ser10.
Molecular Weight:
~17 kDa
Epitope:
Phospho Ser10
Immunogen:
Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, phosphorylated on Ser10, conjugated to KLH.
Modifications:
Phosphorylation
Clone:
CMA312
Isotype:
IgG1
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected, based on sequence homology.
Application Notes:
ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Multiplexing:
This antibody specifically recognizes histone H3 phosphorylated on Ser10 by Luminex® assay.
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Control:
HeLa Acid extract lysate
Quality Assurance:
Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.
Purification Method:
Protein G chromatography
Presentation:
Purified mouse monoclonal IgG in PBS with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P84243
Entrez Gene Number:
NP_003484.1
Gene Symbol:
H3.3A
H3.3B
H3F3
MGC87782
MGC87783
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