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Anti-Acetyl Histone H3 (Lys27), clone CMA309
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REFERENCES
Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis.
Karantzal, Efthimia, et al. (2008) Genome Biol., 9: R65 (2008)
The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
Kimura, Hiroshi, et al. (2008) Cell Struct. Funct., 33: 61-73 (2008)
Alterations of lysine modifications on the histone H3 N-tail under drought stress conditions in Arabidopsis thaliana.
Kim, Jong-Myong, et al. (2008) Plant Cell Physiol., 49: 1580-8 (2008)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt ELISA, ICC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Acetyl Histone H3 (Lys27) Antibody, clone CMA309
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Specificity:
This antibody recognizes Histone H3 acetylated at Lys27. Phosphorylation of Ser28 interferes with antibody binding to acetyl Lys271.
Molecular Weight:
approx. 17 kDa
Epitope:
Acetyl Lys27
Immunogen:
Synthetic peptide corresponding to amino acids 18-36 of human Histone H3, acetylated on Lys27, conjugated to KLH.
Modifications:
Acetylation
Clone:
CMA309
Isotype:
IgG1κ
Background Information:
Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of H3Lys27 is a mark of active chromatin, while histone deacetylase inhibition correlates with gene silencing via repressive Lys27 trimethylation of histone H3.
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected, based on sequence homology.
Application Notes:
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
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Control:
HeLa acid extract lysate, treated and untreated with sodium butyrate.
Quality Assurance:
Western Blot Analysis:
1:10,000 dilution of this antibody detected Histone H3 on 10 μg of HeLa acid extract.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q16695
Entrez Gene Number:
NM_003493
Gene Symbol:
H3.4
H3/g
H3/t
H3FT
H3T
H3t
MGC126886
MGC126888
OTTHUMP00000037945
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Alternate Names:
H3K27Ac
Histone H3 (acetyl K27)
H3 histone family, member T
histone 3, H3
histone cluster 3, H3
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s) |