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Anti-PHD3, clone 188e
Species Reactivity Key Applications Host Format Antibody Type
H WB Mouse Purified Monoclonal Antibody
Description:
Anti-PHD3 Antibody, clone 188e
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Specificity:
This antibody recognizes PHD3.
Molecular Weight:
~ 27 kDa
Epitope:
Unknown
Immunogen:
Recombinant protein corresponding to human PHD3.
Clone:
188e
Isotype:
IgG1κ
Background Information:
PHD3 (Prolyl hydroxylase domain-containing protein 3), also known as Hypoxia-inducible factor prolyl hydroxylase 3 (HIF-PH3 or HPH-1), is one of 4 PHD (PHD1-4) proteins which function as prolyl hydroxylases to effect oxygen homeostasis via hypoxia-inducible factor (HIF). This transcriptional complex is composed of an alpha-beta heterodimer that mediates a broad range of cellular and systemic responses to hypoxia. The PHD proteins hydroxylate HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. PHD3, is expressed in response to hypoxia and causes apoptosis in oxygenated conditions in neural cells. When oxygen levels are normal, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha. This post-translational modification targets this protein for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Control:
Human placenta tissue lysate
Quality Assurance:
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PHD3 on 10 µg of human placenta tissue lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9H6Z9
Entrez Gene Number:
NP_071356.1
Gene Symbol:
EGLN3
HIF-PH3
HIFPH3
HPH-1
PHD3
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Alternate Names:
EGL nine (C.elegans) homolog 3
HIF prolyl hydroxylase 3
HIF-prolyl hydroxylase 3
Hypoxia-inducible factor prolyl hydroxylase 3
Prolyl hydroxylase domain-containing protein 3
egl nine homolog 3
egl nine homolog 3 (C. elegans)
egl nine-like protein 3 isoform
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism By similarity.
CATALYTIC ACTIVITY: An HIF alpha chain L-proline + 2-oxoglutarate + O2 = An HIF alpha chain trans-4-hydroxy-L-proline + succinate + CO2.
COFACTOR: Binds 1 Fe2+ ion per subunit.
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