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Anti-PHD2, clone 76a
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REFERENCES
Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions.
Rotili, Dante, et al. (2011) Chem. Commun. (Camb.), 47: 1488-90 (2011)
Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.
Appelhoff, Rebecca J, et al. (2004) J. Biol. Chem., 279: 38458-65 (2004)
Species Reactivity Key Applications Host Format Antibody Type
H WB Mouse Purified Monoclonal Antibody
Description:
Anti-PHD2 Antibody, clone 76a
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Specificity:
This antibody recognizes PHD2.
Molecular Weight:
~ 46 kDa
Epitope:
Unknown
Immunogen:
Recombinant protein corresponding to human PHD2.
Clone:
76a
Isotype:
IgG1κ
Background Information:
PHD2 (Prolyl hydroxylase domain-containing protein 2), also known as Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2 or HPH-2), is one of 4 PHD (PHD1-4) proteins which function as prolyl hydroxylases to effect oxygen homeostasis via hypoxia-inducible factor (HIF). This transcriptional complex is composed of an alpha-beta heterodimer that mediates a broad range of cellular and systemic responses to hypoxia. The PHD proteins hydroxylate HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. The PHD2 isoform is the main down-regulator of HIFs for normal oxygen levels and mild hypoxia. When oxygen levels are normal, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha. This post-translational modification targets this protein for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Control:
MCF-7 cell lysate
Quality Assurance:
Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 2 µg/ml of this antibody detected PHD2 on 10 µg of MCF-7 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9GZT9
Entrez Gene Number:
NP_071334.1
Gene Symbol:
EGLN1
C1orf12
ECYT3
HIF-PH2
HIFPH2
HPH-2
HPH2
PHD2
SM-20
SM20
ZMYND6
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Alternate Names:
EGL nine (C.elegans) homolog 1
HIF prolyl hydroxylase 2
HIF-prolyl hydroxylase 2
Hypoxia-inducible factor prolyl hydroxylase 2
Prolyl hydroxylase domain-containing protein 2
egl nine homolog 1
egl nine homolog 1 (C. elegans)
egl nine-like protein 1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation f |