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Anti-Bmi-1, clone DC9
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REFERENCES
Association between BMI-1 expression, acute graft-versus-host disease, and outcome following allogeneic stem cell transplantation from HLA-identical siblings in chronic myeloid leukemia.
Mohty, Mohamad, et al. (2008) Blood, 112: 2163-6 (2008)
The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells.
Bracken, Adrian P, et al. (2007) Genes Dev., 21: 525-30 (2007)
Targeting of the Bmi-1 oncogene/stem cell renewal factor by microRNA-128 inhibits glioma proliferation and self-renewal.
Godlewski, Jakub, et al. (2008) Cancer Res., 68: 9125-30 (2008)
Correlation of Bmi-1 expression and telomerase activity in human ovarian cancer.
Zhang, F B, et al. (2008) Br. J. Biomed. Sci., 65: 172-7 (2008)
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Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP Mouse Ascites Monoclonal Antibody
Description:
Anti-Bmi-1 Antibody, clone DC9
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Specificity:
This antibody recognizes murine BMI-1 (~35 kDa).
Molecular Weight:
approx. 35 kDa
Immunogen:
Recombinant fusion protein of full length murine BMI-1 tagged with MBP.
Clone:
DC9
Isotype:
IgG1κ
Background Information:
The Bmi-1 protein is a member of the polycomb group (PcG) proteins. Bmi-1 has been reported as an oncogene by regulating the cell cycle inhibitors p16 and p19. Bmi-1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi-1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi-1 may regulate the self-renewal of other types of somatic stem cells, and it is also thought to inhibit aging in neurons through the suppression of p53. Several lines of evidence implicate Bmi-1 in tumorigenesis. Differential expression of Bmi-1 in a mouse model of metastatic prostate cancer vs. wild-type suggests Bmi-1 expression levels may predict response to therapy and survival in multiple types of cancer.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse. Not tested in other species.
Application Notes:
Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to be suitable for immunoprecipitation1.
Control:
U20S Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on U20S cell lysate.
Western Blot Analysis:
1:5,000 of this lot detected BMI-1 on 15 μg/mL of U20S cell lysate.
Purification Method:
Protein G purfied
Presentation:
Mouse monoclonal IgG1κ ascites in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P35226
Entrez Gene Number:
NP_031578.2
Gene Symbol:
MGC12685
PCGF4
RNF51
flvi-2/bmi-1
Alternate Names:
B lymphoma Mo-MLV insertion region 1 homolog
B lymphoma Mo-MLV insertion region 1 homolog (mouse)
BMI1 polycomb ring finger oncogene
Polycomb group RING finger protein 4
RING finger protein 51
murine leukemia viral (bmi-1) oncogene homolog
oncogene BMI-1
polycomb group ring finger 4
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