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Anti-BMI1, clone AF27
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Species Reactivity Key Applications Host Format Antibody Type
H IP, ChIP Mouse Purified Monoclonal Antibody
Description:
Anti-BMI1 Antibody, clone AF27
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Specificity:
This antibody recognizes BMI1.
Molecular Weight:
~35 kDa
Immunogen:
MBP-tagged recombinant protein corres-ponding to full length human BMI1.
Clone:
AF27
Isotype:
IgG1κ
Background Information:
The BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Application Notes:
Chromatin Immunoprecipitation (ChIP):
A previous lot was used by an independent laboratory in ChIP. (Bracken, A.P., et al., 2007).
Control:
MEF1 cell lysate
Quality Assurance:
Evaluated by Immunoprecipitation in MEF1 cell lysate.
Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated BMI1 from 100 μg of MEF1 cell lysate.
Purification Method:
Anti-BMI1, clone AF27
Presentation:
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable at 2-8°C for 1 year from date of receipt.
UniProt Number:
P35226
Entrez Gene Number:
NP_005171
Gene Symbol:
MGC12685
PCGF4
RNF51
flvi-2/bmi-1
Alternate Names:
B lymphoma Mo-MLV insertion region 1 homolog
B lymphoma Mo-MLV insertion region 1 homolog (mouse)
BMI1 polycomb ring finger oncogene
Polycomb group RING finger protein 4
RING finger protein 51
murine leukemia viral (bmi-1) oncogene homolog
oncogene BMI-1
polycomb group protein Bmi1
polycomb group ring finger 4
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Chromatin Immunoprecipitation (ChIP)
UniProt Summary:
FUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 |