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Anti-EED, clone AA19 Antibody
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REFERENCES
The polycomb group protein Suz12 is required for embryonic stem cell differentiation.
Pasini, Diego, et al. (2007) Mol. Cell. Biol., 27: 3769-79 (2007)
Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions.
Bracken, Adrian P, et al. (2006) Genes Dev., 20: 1123-36 (2006)
EZH2 is downstream of the pRB-E2F pathway, essential for proliferation and amplified in cancer.
Bracken, Adrian P, et al. (2003) EMBO J., 22: 5323-35 (2003)
Polycomb group proteins in cell cycle progression and cancer.
Pasini, Diego, et al. (2004) Cell Cycle, 3: 396-400 (2004)
Species Reactivity Key Applications Host Format Antibody Type
H, M WB Mouse Ascites Monoclonal Antibody
Description:
Anti-EED Antibody, clone AA19
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Specificity:
This antibody recognizes mouse EED, (~30-65 kDa).
Molecular Weight:
Ranges from 30-65 kDa (5-6 isoforms of EED are detected).
Immunogen:
Recombinant fusion protein of full length murine EED tagged with MBP.
Clone:
AA19
Isotype:
IgG2aκ
Background Information:
Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse. Not tested in other species.
Control:
A431 Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on A431cel lysatel.
Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Mouse monoclonal IgG2aκ ascites in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
O75530
Entrez Gene Number:
NP_068676
Gene Symbol:
HEED
WAIT-1
WAIT1
hEED
Alternate Names:
WD protein associating with integrin cytoplasmic tails 1
embryonic ectoderm development
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PR |