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Anti-EZH2, clone BD43 Antibody
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REFERENCES
Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2.
Pasini, Diego, et al. (2008) Genes Dev., 22: 1345-55 (2008)
The polycomb group protein Suz12 is required for embryonic stem cell differentiation.
Pasini, Diego, et al. (2007) Mol. Cell. Biol., 27: 3769-79 (2007)
The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells.
Bracken, Adrian P, et al. (2007) Genes Dev., 21: 525-30 (2007)
Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions.
Bracken, Adrian P, et al. (2006) Genes Dev., 20: 1123-36 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H, M WB Mouse Purified Monoclonal Antibody
Description:
Anti-EZH2 Antibody, clone BD43
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Specificity:
This antibody recognizes human EZH2 (~98kDa).
Molecular Weight:
~98 kDa
Immunogen:
Recombinant fusion protein of amino acids 1-286 of human EZH2 tagged with MBP.
Clone:
BD43
Isotype:
IgG1κ
Background Information:
EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, and mouse. Not tested in other species.
Control:
HeLa Nuclear Extracts
Quality Assurance:
Western Blot Analysis: 1:5,000 from a previous lot detected EZH2 of HeLa Nuclear extract.
Purification Method:
Protein G purfied
Presentation:
Mouse monoclonal IgG1κ ascites in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q15910
Entrez Gene Number:
NP_004447.2
Gene Symbol:
EZH2
KMT6
Alternate Names:
Enhancer of zeste homolog 2
Lysine N-methyltransferase 6
enhancer of zeste 2
enhancer of zeste homolog 2
ENX-1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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