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Anti-SUZ12, clone 2AO9
Species Reactivity Key Applications Host Format Antibody Type
H, Rb IP, ICC, ChIP Mouse Purified Monoclonal Antibody
Description:
Anti-SUZ12 Antibody, clone 2AO9
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Specificity:
This antibody recognizes SUZ12.
Molecular Weight:
~ 95 kDa
Epitope:
Unknown
Immunogen:
Recombinant protein corresponding to human SUZ12.
Clone:
2AO9
Isotype:
IgG
Background Information:
SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. This protein is component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
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Species Reactivity:
Human
Rabbit
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with rabbit based on 100% sequence homology.
Application Notes:
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.
Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChiP. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.
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Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Immunoprecipitation in HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg/mL of this antibody immunoprecipitated SUZ12 in HeLa cells.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q15022
Entrez Gene Number:
NP_056170.2
Gene Symbol:
SUZ12
CHET9
JJAZ1
Alternate Names:
ChET 9 protein
Chromatin precipitated E2F target 9 protein
Joined to JAZF1 protein
Suppressor of zeste 12 protein homolog
joined to JAZF1
suppressor of zeste 12 homolog (Drosophila)
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Immunocytochemistry
Chromatin Immunoprecipitation (ChIP)
Entrez Gene Summary:
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq]
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UniProt Summary:
FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not require |