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Anti-phospho (Ser10) acetyl (Lys14) Histone H3, clone 10H10.2
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PRODUCT FAMILY INFORMATION
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho (Ser10) acetyl (Lys14) Histone H3 Antibody, clone 10H10.2
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Specificity:
This antibody recognizes Histone H3 phosphorylated at Ser10 and acetylated at Lys14.
Molecular Weight:
~ 15 kDa
Epitope:
Ser10 and Lys14
Immunogen:
KLH-conjugated linear peptide corresponding to Histone H3 phosphorylated at Ser10 and acetylated at Lys14.
Modifications:
Phosphorylation
Acetylation
Clone:
10H10.2
Isotype:
IgG1κ
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. The dual modification of histone H3 phosphorylated at serine 10 and acetylated at lysine 14 has been found at genomic sites with active transcription.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human. Broad species cross-reactivity expected.
Application Notes:
Peptide Block Analysis:
To demonstrate specificity, 0.5 µg/ml of this antibody was tested against blocking and control peptides incubated with 10 µg of Colcemid treated HeLa acid extract.
Control:
Colcemid treated and untreated HeLa acid extract
Quality Assurance:
Evaluated by Western Blot in Colcemid treated and untreated HeLa acid extract.
Western Blot Analysis: 0.001 µg/ml of this antibody detected Histone H3 on 10 µg of Colcemid treated and untreated HeLa acid extract.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P84243
Entrez Gene Number:
NP_002098.1
Gene Symbol:
HIST3H3
H3FT
H3t
H3T
H3/g
H3.4
H3/t
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Alternate Names:
H3S10PK14Ac
Histone H3 (phospho S10, acetyl K14)
H3 histone family, member T
histone 3, H3
histone cluster 3, H3
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial us |