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Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4
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REFERENCES
Lys63-linked polyubiquitin chains: linking more than just ubiquitin.
Kawadler, Holli and Yang, Xiaolu (2006) Cancer Biol. Ther., 5: 1273-4 (2006)
Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein.
Galan, J M and Haguenauer-Tsapis, R (1997) EMBO J., 16: 5847-54 (1997)
Species Reactivity Key Applications Host Format Antibody Type
Vrt ELISA, IHC, ICC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4
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Specificity:
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
Molecular Weight:
varies
Epitope:
Lys63 ubiquitination site
Immunogen:
Immunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST).
Clone:
HWA4C4
Isotype:
IgG2a
Background Information:
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
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Species Reactivity:
Vertebrates
Species Reactivity Note:
Human. Based on sequence homology, broad species cross-reactivity is expected.
Application Notes:
Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Additional Research Applications
Immunocytochemistry:
This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.
Immunohistochemistry:
This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer's patient.
ELISA:
This antibody has been reported by an outside laboratory to be suitable for ELISA.
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Control:
Lys63 polyubiquitin chains.
Quality Assurance:
Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Presentation:
Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
P62988
Entrez Gene Number:
NM_018955.2
Gene Symbol:
UBB
UBC
FLJ25987
MGC8385
OTTHUMP00000064960
OTTHUMP00000064961
UBA80
UBCEP1
UBCEP2
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Alternate Names:
ubiquitin C
polyubiquitin B
ubiquitin B
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unau |