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Anti-Ubiquityl-Histone H2B Antibody, clone 56
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REFERENCES
The deubiquitinating enzyme USP36 controls selective autophagy activation by ubiquitinated proteins.
Taillebourg, Emmanuel, et al. (2012) Autophagy, 8: (2012)
The interaction of the von Hippel-Lindau tumor suppressor and heterochromatin protein 1.
Yanlai Lai,Meihua Song,Kevin Hakala,Susan T Weintraub,Yuzuru Shiio (2012) Archives of biochemistry and biophysics.518
Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation.
Weake, Vikki M, et al. (2011) Genes Dev., 25: 1499-509 (2011)
Detection and characterization of ubiquitylated H2B in mammalian cells.
Efrat Shema,Moshe Oren,Neri Minsky (2011) Methods (San Diego, Calif.).54
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Mouse Purified Monoclonal Antibody
Description:
Anti-Ubiquityl-Histone H2B Antibody, clone 56
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes histone H2B when it is ubiquitinated at Lys120 but not H2B in its unmodified state, or ubiquitin unconjugated to H2B. Mr ~25 kDa.
Molecular Weight:
~25 kDa
Epitope:
Ubiquityl Lys120
Immunogen:
Immunogen used was a branched peptide corresponding to the ubiquitination site on human H2B.
Modifications:
Ubiquitylation
Clone:
clone 56
Isotype:
IgG2aκ
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression of several yeast genes.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Based on sequence homology, broad species cross-reactivity is expected.
Control:
HeLa cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot
Western Blot Analysis:
A 0.25 μg/mL dilution of a recent lot detected ubiquityl histone H2B in HeLa cells.
Purification Method:
Protein G purfied
Presentation:
Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
P62807
Entrez Gene Number:
NP_066406.1
Alternate Names:
H2BUb
Histone H2B (ubiquityl)
HIST1H2B
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chro |