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Anti-Ubiquitin, Lys63-Specific,
clone Apu3
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REFERENCES
mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6.
Nazio, Francesca, et al. (2013) Nat. Cell Biol., 15: 406-16 (2013)
Proteomic identification and analysis of K63-linked ubiquitin conjugates.
Cannon, Joe, et al. (2012) Anal. Chem., 84: 10121-8 (2012)
De-ubiquitinating protease USP2a targets RIP1 and TRAF2 to mediate cell death by TNF.
Mahul-Mellier, A-L, et al. (2011) Cell death and differentiation, (2011)
Bifunctional apoptosis regulator (BAR), an endoplasmic reticulum (ER)-associated E3 ubiquitin ligase, modulates BI-1 protein stability and function in ER Stress.
Juan Rong,Lili Chen,Julia I Toth,Marianna Tcherpakov,Matthew D Petroski,John C Reed (2011) The Journal of biological chemistry.286
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Rb Cell Function Assay, ICC, IP, IHC, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3, rabbit monoclonal
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Product Overview:
DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys63-linked) Recombinant Protein (17-10388)
Specificity:
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
Molecular Weight:
varies
Epitope:
Lys63 ubiquitination site
Immunogen:
Immunogen used was human di-ubiquitin linked at Lys63.
Clone:
Apu3
Isotype:
IgG
Background Information:
Polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
In contrast, polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
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Species Reactivity:
Human
Mouse
Rat
Rabbit
Species Reactivity Note:
Human, mouse, rabbit, rat. Based on sequence homology, broad species cross-reactivity is expected.
Application Notes:
Immmunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-63-specific), clone Apu3, precipitated ubiquitin-containing proteins from HeLa cell lysates
Immunohistochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells.
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys63-specific (#05-1308) (blue) and rabbit IgG control (red).
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys63-specific, with K63-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Immunocytochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat, and rabbit brain sections.
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Control:
Lys63 polyubiquitin chains.
Quality Assurance:
Western Blot Analysis:
A 1:1000 dilution of this antibody detected Lys-63 ubiquitin chains by Western blotting, but did not detect free ubiquitin or Lys48 polyubiquitin chains.
Purification Method:
Protein A purfied
Presentation:
Protein A purified recombinant rabbit monoclonal antibody that |